Cell-free gene expression

Cell-free gene expression (CFE) emerged as an alternative approach to living cells for specific applications in protein synthesis and labelling structural biology proteomics studies. CFE has since been repurposed a versatile technology synthetic bioengineering. However, taking full advantage of this requires in-depth understanding its fundamental workflow beyond existing protocols. This Primer provides new practitioners with comprehensive, detailed actionable guide best practices CFE, inform research the laboratory at state art. We focus on Escherichia coli-based systems, which remain primary platform efficient CFE. Producing proteins, biomanufacturing therapeutics, developing sensors prototyping genetic circuits illustrate broader utility opportunities provided by practical introduction With extensive functionality portability, is becoming powerful enabling tool biotechnology. useful expressing proteins post-translational modifications, special folding requirements whose difficult prokaryotic systems. Garenne et al. outline cell-free environment.